RNASure® Virus Kit

Code: NP-67705/NP-67709

Pack Size: 50 reaction, 250 reaction


RNASure Virus kits are designed for the rapid preparation of highly pure viral nucleic acids (e.g. HCV, HIV, CMV) from fluid biological samples e.g. plasma, serum, urine, but not blood. RNASure Virus kit is intended for general laboratory use. RNA viruses are lysed by Lysis Buffer VLB1 whereas, DNA viruses which are relatively more difficult to lyse require Proteinase K digestion. Addition of Carrier RNA improves binding and recovery of low concentrated viral RNA. The RNA binds to the membrane and contaminants (potential PCR inhibitors) like salts, metabolites and soluble macromolecular cellular components are removed efficiently in two wash steps using two different buffers VWB and VWB3. The nucleic acids can be eluted in low salt buffer or water (RNase free) and are ready-for-use in subsequent downstream applications like PCR, real time PCR etc. High-quality RNA is eluted in a special RNase-free buffer, ready for direct use or safe storage. The purified RNA is free of any contaminants and inhibitors.


Features and Benefits:

Format: Spin column based; Processing Time: 30 min (4-6 prep); Sample Input: 150 ul serum, plasma and cell free biologicals; Elution Volume: 50ul . DNA Purity: A260/280 ratio 1.7-1.9. RNA Yield: 40 ug.