RNASure® Mini Kit

Code: NP-84105 / NP-84109

Pack Size: 50 reaction, 250 reaction


RNASure® Mini kit is simple reliable and rapid method for the isolation of total RNA from cultured cells and tissues. With the help of supplementary protocol total RNA can be isolated from cell-free biological fluids, bacteria and yeast using RNASure® Mini kits. If used in RT-PCR applications we recommend using intron-spanning primers. RNA purified can be used in further downstream applications like RT-PCR, qRT-PCR, Northern Blotting, cDNA Synthesis, or RNase protection assays. Even difficult biological materials like mouse tissue (liver, brain), different tumor cell lines, Streptococci and Actinobacillus pleuropneumoniae will give high quality of RNA. RNA isolated with RNASure® Mini gives high integrity. The RNA Integrity number of RNA isolated from fresh high quality sample material generally exceeds 9.0. However, RNA Integrity majorly depends on the sample quality. The RNASure® Mini kit is supplied with rDNase for an On column digestion to have minimal contamination.


Features and Benefits:

Format: spin column based; Processing Time: 30 min; Sample Input: 30mg of tissue and 100 ul of serum, Elution Volume: 60 ul. RNA Purity: A260/280 ratio 1.9. RNA Yield: 70 ug.