N-glycan analysis has become a CQA for a number of therapeutic proteins. The N-glycan kit by Genetix is used for analysis of N-glycans present in mAbs by fast enzymatic release by PNGase F and rapid labeling by MS reagent. The labeled N-glycans can then be analyzed on UPLC-FLR based chromatographic system. The label is MS compatible, therefore, the N-glycan peaks can be easily characterized on LC-MS. The kit has reduced the sample preparation time from days in conventional methods to minutes. It can be used for analysis of all proteins containing N-glycans.
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N-glycan kit #HCP0031A
N-glycan analysis has become a CQA for a number of therapeutic proteins. The N-glycan kit by Genetix is used for analysis of N-glycans present in mAbs by fast enzymatic release by PNGase F and rapid labeling by MS reagent. The labeled N-glycans can then be analyzed on UPLC-FLR based chromatographic system. The label is MS compatible, therefore, the N-glycan peaks can be easily characterized on LC-MS. The kit has reduced the sample preparation time from days in conventional methods to minutes. It can be used for analysis of all proteins containing N-glycans.
| Module | Model 1 (24T/kit) | Model 2 (96T/kit) | ||
| Component | Storage | Component | Storage | |
| Deglycosylation
Module (HCP0031A-1) |
IgG (1.2 mg) | 2-8℃，12 months | IgG (5 mg) | 2-8℃，12 months |
| PNGase F (45 µL) | -25℃ ～ -15， 12 months， avoid
freeze/thaw cycles |
PNGase F (150 µL) | -25℃ ～ -15， 12 months， avoid
freeze/thaw cycles |
|
| PNGase F buffer (0.5 mL) | 2-8℃，12 months | PNGase F buffer (1.0 mL) | 2-8℃，12 months | |
| Surfactant (1.2 mg*6) | Room Temperature, 12 months | Surfactant (3.6 mg*8) | Room Temperature, 12 months | |
| Labeling Module (HCP0031A-2) | MS reagent powder (8.2 mg*3) | 2-8℃，12 months, keep in dark | MS reagent powder (24.6 mg*4) | 2-8℃，12 months, keep in dark |
| Anhydrous DMF (1 mL) | Room temperature, 12 months | Anhydrous DMF (1 mL) | Room temperature, 12 months | |
| Clean-up Modue (HCP0031A-3) | Elution Plate (96 well) | Room temperature, once opened, the
unused well need to be sealed with the plastic paraffin film and keep desiccant at room temperature |
Elution Plate (96 well) | Room temperature, once opened, the
unused well need to be sealed with the plastic paraffin film and keep desiccant at room temperature |
| Elution Buffer (5 mL*1) | Room temperature, 12 months | Elution Buffer (5 mL*4) | Room temperature, 12 months | |
| Sample Diluent (5 mL*1) | Room temperature, 12 months | Sample Diluent (5 mL*4) | Room temperature, 12 months | |
| Sample Collection Module (HCP0031A-4) | 8-tube Strips (200 µL) (100 pieces) | Room temperature, 96 well collection
plate be sealed and keep desiccant at room temperature |
8-tube Strips (200 µL) (100 pieces) | Room temperature, 96 well collection
plate be sealed and keep desiccant at room temperature |
| 8-cap Strips (100 pieces) | 8-cap Strips (100 pieces) | |||
| Collection plate(96 well) | Collection plate(96 well) | |||
| Waste tray | Waste tray | |||