FAST, REPRODUCIBLE AND SAFE MEDIA STERILIZATION
Preparing high quality media for the cultivation of bacteria is crucial to various routine applications in today’s laboratories, and many downstream tasks and results depend directly on the quality of that media. Furthermore, efficient media production is important to reduce labor costs and free staff time for less repetitive tasks.
The MEDIACLAVE product range is designed to address these needs by offering rapid and gentle formulation and sterilization of up to 30 liters of culture media. Precise control and monitoring of temperature, time and pressure during the sterilization process guarantees constant high quality. The intuitive graphical user interface and simple programming make it very easy for everyone to operate MEDIACLAVE.
MEDIACLAVE allows you to be flexible, by providing high quality culture media whenever it is needed. This minimizes the need to store media, saving space and eliminating concerns about short shelf lives to guarantee the availability of high quality media.
MEDIUM FORMULATION
MEDIACLAVE is quick to set up. Insert the cuvette (media container), fill the water jacket between the cuvette and the vessel to ensure efficient heat transfer, and you are ready to prepare your culture media. Culture media can be suspended and dissolved directly within MEDIACLAVE. The strong magnetic stirrer guarantees homogeneous mixing within the vessel and prevents coagulation. Alternatively, culture media can be dissolved and pre-swelled using the WATER BATH mode prior to sterilization. The intuitive, multilingual graphical user interface makes it very easy for everyone to use MEDIACLAVE – no special training is required. Up to 50 programs with user-defined parameters – such as sterilization temperature, dispense temperature and sterilization time – can be saved for later use.
Operation modes
Two main operation modes for media preparation, plus two extra modes can be selected and the parameters set
to your needs:
• STANDARD: For the preparation of standard and highly sensitive culture media. Sterilization temperature/ time and dispensing temperature can be adjusted.
• CHOCOLATE AGAR: This two-step program allows the preparation of complex media. After the first sterilization phase, a supplement (e.g. blood) can be added through a separate port, followed by a second heating phase.
• WATER BATH: For pre-swelling culture media prior to sterilization, with a temperature range of 30-80 °C. In combination with an autoclave cuvette, this mode can be used to thermostat liquids in glassware (MEDIACLAVE 10 only).
• AUTOCLAVE: With the optional autoclave kit, the MEDIACLAVE 10 can be used as a benchtop autoclave for the sterilization of small amounts of culture media in glassware such as Erlenmeyer flasks or test tubes.
MEDIUM STERILIZATION
MEDIACLAVE working principle
1 Media cuvette
2 Sterilization vessel
3 Temperature probe
4 Adding port
5 Overpressure safety valve
6 Dispense port with decanting tubing
7 Lid seal
8 Magnetic stirrer bar
9 Sterile filter
10 Support pressure pump
11 Flow heater
12 Deaeration outlet
13 Air inlet
14 Quick connection for cooling water inlet
15 Heat exchanger
16 Quick connection for water outlet
17 Quick connection for coupling water
inlet (MEDIACLAVE 30 only)
18 Circulation pump
Valve
MEDIACLAVE guarantees gentle and rapid sterilization of both standard and highly sensitive culture media. An efficient heating and cooling system – together with homogeneous mixing – minimizes thermal stress during the process, maximizing media quality.
DISPENSING OF THE STERILIZED MEDIA
The wide adding port enables fast and safe addition of supplements – such as antibiotics, blood or growth factors – prior to dispensing. Automatic, in-process sterilization of the dispense port eliminates the risk of media contamination during dispensing. The vessel and dispensing system of the MEDIACLAVE are designed to minimize dead volumes and maximize yields, allowing laboratories to reduce costs. The MEDIACLAVE can be quickly and conveniently connected to the dispensing tubing of the MEDIAJET – an automated Petri dish filler – to dispense up to 540 Petri dishes of agar media at the touch of a button
High Operational Safety
An automatic pre-sterilization tightness check ensures the vessel is properly sealed before starting, avoiding incomplete
sterilization and subsequent media rejection. Furthermore, MEDIACLAVE offers multiple independent pressure and temperature monitoring systems, guaranteeing user and environmental safety. The vessel lid is also equipped with an autonomous overpressure safety valve and burst disc in case other electronic monitoring systems fail.
Fast Heating
Powerful heating elements permit fast media processing, minimizing thermal stress and ensuring culture media remains highly effective. Pressure- and temperature controlled deaeration guarantees saturated steam in the vessel for complete sterilization.
Reliable Sterilization
The large, strong magnetic stirrer offers adjustable speed and reversing rotation direction to ensuring homogeneous mixing across different viscosities. Process parameters are controlled by a Pt-1000 temperature probe and microprocessor for reliable and complete sterilization of culture media.
Rapid Cooling
An efficient plate heat exchanger and built-in support pressure system enable rapid, gentle cooling, and the isolated cooling water system prevents media contamination.
Versatile dispensing
The DOSE IT peristaltic pump can be used to fill containers with unusual volumes or shapes, such
as quadrangular Petri dishes, bottles or flasks. Alternatively, the pressure dispensing kit – including a pinch valve box and a foot switch – allows fast, direct dispensing of media into large containers (e.g. bottles) by increasing the pressure inside the sterilization chamber.
Download:- Mediaclave 10/30 Brochure