GenezolTM LS RNA Extraction Reagent, 100 ml (for liquid samples) #PG-100203

Genezol LS RNA Extraction Reagent is a complete kit with ready-to-use reagents for isolation of total RNA from various liquid samples. Genezol LS is a concentrated form that allows the liquid samples to be processed more easily. Genezol LS is a mono-phasic solution containing phenol and guanidine salt which rapidly lyse cells and inactivates nucleases. Addition of chloroform brings about a separation of the homogenate in aqueous and organic phases. RNA locates in the aqueous phase while DNA and protein remain in the interphase and organic phases. The aqueous phase including RNA is mixed with isopropanol and the RNA is precipitated by centrifuging. The purified total RNA is suitable for RT-PCR, northern blotting, dot blotting, in vitro translation, molecular cloning, real-time PCR, RNase protection assays and other analytical procedures.