With the DNASure Tissue Mini Kit method genomic DNA can be prepared from tissue, cells (e.g., bacteria), and many other sources. Lysis is achieved by incubation of the sample material in a proteinase K / SDS solution. Appropriate condition for binding of DNA to the silica membrane in the DNASure Tissue Mini Kit Columns is achieved by the addition of chaotropic salts and ethanol to the lysate. The binding process is reversible and specific to nucleic acids. Contaminations are removed by subsequent washing with two different buffers. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer.
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DNASure Tissue Mini Kit (50 Preps) #NP-61305
- Extracts genomic DNA from tissue, cells, and bacteria.
- Lysis done using proteinase K/SDS solution.
- DNA binds to silica membrane with chaotropic salts and ethanol.
- Contaminants removed by washing with two different buffers.
- Elutes pure DNA in slightly alkaline buffer; yields 15-35 µg.
Format: Mini spin column
Sample amount :< 20 mg Human / Animal Tissue, < 25 mg human / Animal Tissue,
10 –10 Cultured Cells
Fragment size: 100 bp–approx. & 60 kbp
Elution volume: 60–200 µL 50-200 µL
Typical yield: 20–35 µg 15-30 µg