Ni-NTA products enable efficient and quick purification of recombinant polyhistidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC). Proteins can be purified under native or denaturing conditions from any expression system. Binding is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions. Ni-NTA Agarose consists of the chelating ligand nitrilotriacetic acid (NTA) immobilized on 6 % cross-linked agarose beads that are suitable for batch binding, gravity flow, and Fast protein liquid chromatography (FPLC) columns.
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Ni-NTA Agarose, 500ml #NP-40251
- High-quality, stable and resilient affinity support
- Bind up to 50 mg of 6xHis-tagged protein per ml of resin
- Purify proteins using native or denaturing conditions
- Same batch of resin can be reused at least five times