RNASure Fusion miRNA is designed for purification of large and small RNA separately from culture cells or animal tissues, and co-purification in a single tube and is also available by modified protocol. This kit utilizes the lysis method at Buffer ER which has a powerful ability of lysis and the purification method based on glassfiber membrane technology. Total RNA locates in the aqueous phase while DNA and protein remain in the interphase and organic phase. Large and small RNA in the aqueous phase is selectively bound to column type B and type W respectively. To purify large RNA, the aqueous phase is mixed with ethanol and the mixture is applied to a column type B. The membrane is washed away by two wash buffer (SW1 and RNW) and purified large RNA is eluted from the membrane by RNase-free water. To purify small RNA, the pass-through come from the binding of large RNA is mixed with ethanol and then applied to a column type W. After washing with buffer RBW and RNW, small RNA is eluted by RNase-free water. The purified RNA is suitable for the isolation of Poly A+ RNA, Northern blotting, dot blotting, in vitro translation, cloning, RT-PCR, RPA and other analytical procedures.
Preparation time: 30 minutes; sample size: upto 50 mg tissue; Recovery range: Large RNA: >200 nucleotide; Small RNA: > 200 Nucleotide; Instant Use: No need of additional materials; No ethanol precipitation; No Genomic DNA contamination.