QuickClone™ PCT Cloning Kit

Code: PGK121

Pack Size: 30 rxn


The Purgene’s QuickClone™ PCR Cloning Kit is a TA system for direct one-step cloning of PCR products with 3’-dA overhangs . The high quality TA cloning vector pTZ57R/T is ready to use for efficient ligation with PCR products providing high cloning yields and low background. To increase the speed, convenience and efficiency of cloning, the QuickClone PCR Cloning Kit has been combined with the Bacterial Transformation Kit – a set of solutions for preparation of chemically competent E. coli cells. According to our protocol, ligation and preparation of competent cells is performed in parallel. Therefore, it only takes approximately one hour from the completion of PCR to plating of transformed cells. Our transformation protocol is often faster than transformation of commercially available competent cells. The DNA insert can be readily excised from the versatile polylinker of pTZ57R/T, sequenced using standard M13/pUC primers or in vitro transcribed with T7 RNA polymerase.


Features and Benefits:

The QuickClone PCR Cloning Kit takes advantage of the terminal transferase activity of Taq DNA polymerase and other non-proofreading thermostable DNA polymerases. Keep the ligation mixture at -20 °C if transformation is postponed.