Protease inhibitors are critical reagents for the preservation of protein integrity during purification and analysis procedures. Inhibitor cocktails are extensively used to provide broad spectrum protection during cell lysis and tissue dissolution. These cocktails target a range of different proteases including serine, cysteine, aspartic and metalloproteases as well as aminopeptidases. Puregene offers four protease inhibitor cocktails designed to preserve the protein composition of samples in a broad range of organisms. A general use cocktail is available with or without EDTA, while specific formulations are available for mammalian, bacterial or plant lysates. All formulations are prepared from ultrapure reagents to ensure maximum inhibition of the specified proteases. Cocktails are provided as convenient, easy-to-use powders that can be reconstituted in the supplied vials.
The lyophilized powder should be stored at -20ºC where it is stable for up to 1 year. The reconstituted solution should be stored at -20ºC where it is stable for up to 2 weeks. Repeated freezing and thawing cycles of the reconstituted solution is not recommended