Ni-NTA Agarose

Code: NP-40211

Pack Size: 25 reaction, 100 reaction, 500 reaction


Ni-NTA products enable efficient and quick purification of recombinant polyhistidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC). Proteins can be purified under native or denaturing conditions from any expression system. Binding is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions. Ni-NTA Agarose consists of the chelating ligand nitrilotriacetic acid (NTA) immobilized on 6% cross-linked agarose beads that are suitable for batch binding, gravity flow, and FPLC columns. NTA is a tetradentate chelator which occupies four out of the six binding sites in the coordination sphere of the Ni2+ ion. The remaining two coordination sites are usually occupied by water molecules and can be exchanged with histidine residues of the recombinant protein. This formation of coordination sites has turned out to be optimal for purification of polyhistidine-tagged proteins: two available binding sites in the coordination sphere of the Ni2+ ion enable tight but reversible selective protein interactions. Chelation of Ni2+ ions by NTA through four coordination positions minimizes metal leaching during purification and increases specificity for poly-histidine tagged proteins.


Features and Benefits:

The product is shipped at room temperature and Upon receipt Ni-NTA Agarose products should be stored at 2- 8 degree Celsius. and are stable up to 1 year. Do not freeze.