GeneSure H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes GeneSure a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA. This is applicable for the first strand cDNA synthesis for RT, construction of full length cDNA libraries, antisense RNA synthesis. The recombinant Riboblock RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with the reverse transcription reaction, as it maintains activity at temperatures up to 55°C.The kit is supplied with both oligo (dT)18 and random hexamer primers. The oligo (dT) 18 primer anneals selectively to the poly (A) tail of mRNA. Random hexamer primers do not required the presence of poly (A), therefore, they can be used for transcription of the 5’- end regions of mRNA or for cDNA synthesis using RNA without the poly(A) tail e.g. micro RNAs. Gene specific primers may also be used with the kit. The first strand cDNA can be directly used as a template
First strand cDNA up to 13 kb; reaction temperatures in the range of 42 to 55°C; Supplied with the recombinant Ribo; Complete kit—oligo(dT)18 and random hexamer primers included with the kit.The first strand cDNA can be directly used as a template in PCR, realtime PCR or in second strand cDNA synthesis.